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Report 2018-03-12T12:21:07+00:00

Bacillus strains used by Organic Pond, LLC for aquaculture pond
treatments were tested for their ability to inhibit the growth of multiple
aquaculture pathogens under laboratory conditions.

Each of the 4 Bacillus strains were observed to produce metabolites that inhibited the growth of pathogenic bacterial or oomycete pathogens.

The antibiosis pattern observed was different for each of the 4 Bacillus strains, which suggests that they each produce a unique metabolite(s) with activity against aquaculture pathogens.

Zone of Inhibition against Aquaculture Pathogens

Pathogen Bacillus subtilis Bacillus licheniformis Bacillus amyloliquefaciens Bacillus pumilus
Edwardsiella ictaluri strain 219 0.3 no inhibition no inhibition 0.1
Edwardsiella tarda strain AL97-38 0.7 0.5 0.2 0.5
Aeromonas hydrophila ML09-119
(virulent strain causing epidemic)
no inhibition 0.8 0.2 0.1
Aeromonas hydrophila AL06-06 0.5 1 0.5 0.5
Vibrio parahaemolyticus isolate 490
(from Ecuador in shrimp)
2 1.2 2 1
Vibrio harveyi isolate 536
(from Ecuador in shrimp)
0.3 no inhibition 2 no inhibition
Steptococcus iniae 0.3 0.3 0.2 0.2
Yersinia ruckeri no inhibition 0.2 0.1 0.1
Saprolegnia ferax 0.5 0.3 0.4 0.3